Table 2 VLX1570 binding to immobilised 26S proteasome (VIVA Biosciences) analysed by surface plasmon resonance (SPR) using Biacore T200 and CM7 chip.

From: The proteasome deubiquitinase inhibitor VLX1570 shows selectivity for ubiquitin-specific protease-14 and induces apoptosis of multiple myeloma cells

Immobilised protein on chip

Average protein load (RU)

VLX1570 binding Relative RU × 100 +/− S.D.

26S proteasome

8500

0.21 ± 0.09

26S proteasome Ub-VS

10850

0.04 ± 0.01

  1. Protein load on chip and relative light units(RU)×100 ± SD (defined as average of the ratio of VLX1570 RU divided by individual protein load RU) are listed. Values are the average of two chip experiments repeated three times.
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