Figure 1

EGFR degradation is delayed in Alix ko cells.

(a,b) Serum-starved wt or Alix ko MEFs were incubated with 100 ng/ml EGF at 37 °C for the indicated times. EGFR was revealed by immunoblotting (a) and band intensities estimated (b) using ImageJ software. EGFR intensities were normalised to actin. Graph represent the ratio between values at each time point and at t = 0 (n = 6; *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test). Dashed lines indicate degradation curves fitted with single exponential functions.