Table 1 Comparison of point scatter above and below the level of detection (LOD) for various analytes and tissues combinations.

Cytokine	Tissue	LOD	N	Above	Below	CV-A	CV-B	Ratio	p.value	Sig.
IFN-g (21)	plasma	17.418	11	80	11	0.025	0.076	0.327	0.001	**
IFN-g (21)	serum	17.418	28	52	28	0.059	0.081	0.724	0.064
IL-10 (56)	plasma	34.966	29	29	62	0.161	0.129	1.248	0.472
IL-10 (56)	serum	34.966	32	48	32	0.116	0.133	0.873	0.319
IL-11 (39)	plasma	27.995	33	33	58	0.14	0.123	1.136	0.945
IL-11 (39)	serum	27.995	37	37	43	1.239	0.146	8.469	0	***
IL-22 (18)	plasma	27.463	12	79	12	0.016	0.092	0.173	0	***
IL-22 (18)	serum	27.463	24	56	24	0.082	0.108	0.759	0.188
IL-27 (13)	plasma	24.529	9	82	9	0.027	0.101	0.271	0.001	**
IL-27 (13)	serum	24.529	23	56	23	0.157	0.155	1.013	0.461
IL-29 (33)	serum	18.187	19	61	19	0.045	0.078	0.574	0.018	*
IL-32 (35)	plasma	58.531	37	37	54	0.262	0.204	1.282	0.867
IL-32 (35)	serum	58.531	39	41	39	0.322	0.284	1.131	0.902
IL-34 (15)	plasma	60.664	16	16	75	0.229	0.177	1.296	0.868
IL-34 (15)	serum	60.664	24	24	56	1.319	0.176	7.509	0	***
Light (51)	plasma	136.28	29	62	29	0.059	0.116	0.505	0.001	**
Light (51)	serum	136.28	8	72	8	0.049	0.106	0.465	0.056

LOD represents the fluorescence level associated with the concentration determined as the lower level of detection, N the number of responses above and below the associated LOD used in the analysis. Above and Below give the actual number of observed responses above and below the LOD. The columns CV-A and CV-B give the coefficient of variation (CV) of the samples, each of size N, above and below the respective LOD. Ratio gives the value of CV-A/CV-B. P.value represents the uncorrected probability of an F test statistic. The number of *’s in the Sig. column indicates the significance of the associated p-values for each analyte and tissue combination.

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