Figure 4
The inhibition on the production of inflammatory mediators, cytokines and ear edema after treatment with the combination and each compound of Esc, Cur and Her at the indicated optimal concentrations.
(A) Cytotoxic effects of the combination of Esc, Cur and Her at the indicated optimal concentrations. Raw264.7 cells were treated with the combination of Esc 11.6 μM, Cur 8.5 μM and Hes 12.5 μM alone or followed by 1 μg·mL−1 LPS for 24 h. Cells were then subjected to MTT assay as described in the Methods. (B–F) The inhibition on the production of PGE2 (B), NO (C), TNF-α (D), IL-1β (E) and IL-6 (F) after treatment with the combination and each compound of Esc, Cur and Her at the indicated optimal concentrations of 11.6 μM, 8.5 μM and 12.5 μM, respectively. (G) The inhibition on croton oil-induced mouse ear edema after treatment with the combination and each compound of Esc, Cur and Her at the indicated optimal dosage of 337 mg·kg−1, 60 mg·kg−1 and 191 mg·kg−1, respectively. All values are presented as the mean ± S.E. and are the result of three independent experiments. *p < 0.05, **p < 0.01 as compared to Esc+ Cur+Hes group. (Esc+ Cur+Hes represent combination of Esc, Cur and Hes).
