Figure 2
Biobanking preserves protein-DNA interaction structure.
(a) Histogram of the distribution of fragment lengths in reads from all fresh or biobanked samples. The enriched regions of sub-nucleosomal (<150 bp) and di-nucleosomal (260–340) are indicated. Histograms of fresh and biobanked reads separated by fragment lengths of (b) <150 bp and (c) 260–340 bp at all hg19 RefSeq transcription start sites (TSS). The vertical bar indicates the position of the TSS. Histograms of fresh and biobanked reads were separated by fragment length of (d) <150 bp and (e) 260–340 bp at 56,208 CTCF motifs. The CTCF motif used for the analysis is shown above the footprint. (f) Histogram comparing fragments corresponding to sub-nucleosomal lengths from fresh and biobanked samples at 11,318 RFX5, 18,094 NYFB, 12,115 CREB1 and 56,420 PU.1 motifs. The motif used for each analysis is indicated. (g) Histogram comparing fragments corresponding to di-nucleosomal reads from fresh and biobanked samples at the transcription factor motif locations described in D.
