Figure 6: The effect of proteasome inhibitor after PanIN formation.
From: Proteasome activity is required for the initiation of precancerous pancreatic lesions
Gdeg;Pdx-1-Cre;LSL-KrasG12D mice were simultaneously treated with two sets of caerulein and MG132 on alternating days (left panels), or first treated with two sets of caerulein and after PanIN formation further treated with two sets of MG132 on day 14 and 16 (right panels). Mice were analyzed on day 28 (n = 3). (a) H&E staining, Alcian blue staining, fluorescent imaging of the pancreas of Gdeg;Pdx-1-Cre;LSL-KrasG12D mice. (b) Quantification of Alcian blue positive areas at day 28. Values are shown as mean ± SD. (c) Immunofluorescence staining for pERK, cyclin D1, NF-κB and cox2 of the pancreas of Gdeg;Pdx-1-Cre;LSL-KrasG12D mice. Dashed lines on the fluorescence images mark PanIN lesions. Bar, 100 μm. (d) Quantification of the positive staining rates of pERK, cyclin D1, NF-κB, and Cox2 in PanIN cells at day 28. Values are shown as mean ± SD.
