Figure 1
From: LPS/TLR4 Signaling Enhances TGF-β Response Through Downregulating BAMBI During Prostatic Hyperplasia
LPS in the presence of TGF-β1 induces changes in expression of EMT-associated markers in BPH-1 cells.
BPH-1 cells were incubated with LPS (100 ng/ml) in the presence or absence of TGF-β1 (0.1 or 10 ng/ml). (A,B) E-Cadherin and vimentin mRNA expression were examined by qPCR. (C) Western blot analysis of E-cadherin and vimentin. Values shown indicate relative levels corrected for β-actin. (D) Immunocytochemical staining of E-cadherin and vimentin in BPH-1 cells after treatment with LPS in the presence or absence of TGF-β1 (0.1 or 10 ng/ml). Scale bar, 50 μm. *P < 0.05, determined by multifactorial ANOVA.
