Figure 3
From: LPS/TLR4 Signaling Enhances TGF-β Response Through Downregulating BAMBI During Prostatic Hyperplasia
Functional activation of the LPS/TLR4 axis mediates BAMBI downregulation.
(A) Immunocytochemical analysis was used to assess the expression of TLR4 and BAMBI in untreated and 100 ng/ml LPS-treated BPH-1 cells. Scale bar, 50 μm. (B) Double immunofluorescent technique was performed to examine the localisation of TLR4 and BAMBI in BPH-1 cells. TLR4 was localised in the cell membrane and cytosol (red) and BAMBI was detected in the membrane, cytoplasm and some nuclei (green). Scale bar, 50 μm. (C) BPH-1 cells were incubated with LPS (100 ng/ml) or pretreated with CLI-095 (3 μmol/L). Total RNA was subjected to qPCR. (D) Western blotting analysis of expression of BAMBI in control and LPS alone or pretreated with CLI-095 (3 μmol/L) for 1 h treated BPH-1 cells. (E) Immunocytochemical analysis of expression and localisation of BAMBI (brown) after LPS treatment or pretreated with CLI-095. Scale bar, 50 μm. *P < 0.05, determined by multifactorial ANOVA.
