Figure 5
From: LPS/TLR4 Signaling Enhances TGF-β Response Through Downregulating BAMBI During Prostatic Hyperplasia
Expression of TLR4, BAMBI, p-Smad2/3 in BPH patient samples.
Prostate specimens obtained by TURP from patients with BPH (n = 62) were stained by H&E. (A) A total of 47 of 62 patients had inflammatory cells infiltrating BPH tissues, while 15 of 62 patients had no obvious inflammatory cellular infiltration in BPH tissues. Scale bar, 100 μm. (B) Prostatic levels of TLR4 mRNA were measured by qPCR in the inflammatory group (n = 47) and non-inflammatory group (n = 15). *P < 0.001. (C) Immunostaining of TLR4. TLR4 (brown staining) was expressed in the cytoplasm in the glandular epithelium cells. The mean TLR4 staining score in the inflammatory group was compared with the non-inflammatory group. Scale bar, 50 μm. *P < 0.001. (D) Prostatic levels of BAMBI mRNA were measured by qPCR in the inflammatory group (n = 47) and non-inflammatory group (n = 15). *P < 0.001. (E) BAMBI (brown staining) was expressed in the cytoplasm and several nuclei of glandular epithelium cells and some stromal cells. The mean BAMBI staining score in the inflammatory group was compared with non-inflammatory group. Scale bar, 50 μm. P = 0.095. (F) P-Smad2/3 was expressed in the nuclei of glandular epithelial cells but not in the basal cells. The mean p-Smad2/3 staining score in the inflammatory group was compared with non-inflammatory group. Scale bar, 50 μm. *P < 0.001, determined by Wilcoxon rank sum test.
