Figure 1 | Scientific Reports

Figure 1

From: Inhibition of herpes simplex virus 1 gene expression and replication by RNase P-associated external guide sequences

Figure 1

Schematic presentation of RNase P substrate/synthesized EGS complex.

(A) A pre-tRNA structure. (B) A hybridized complex of a target RNA and an EGS resembling the T-stem and loop and variable region of a pre-tRNA structure. (C–F) Hybridized complexes of HSV-1 ICP4 mRNA and EGS SER-A, SER-I, C468-A and C468-I, respectively. The sequences of SER-A and SER-I were derived from natural pre-tRNASER, while those of C468-A and C468-I were from EGS variant C468. The targeting sequences of ICP4 mRNA are shown in red and the EGS sequences are shown in blue, respectively. The site of cleavage by RNase P is marked with an arrowhead. The three mutated positions to inactivate EGS activity are marked in black box.

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