Figure 4 | Scientific Reports

Figure 4

From: Inhibition of herpes simplex virus 1 gene expression and replication by RNase P-associated external guide sequences

Figure 4

Expression levels of HSV-1 mRNAs with RNase protection assay.

Parental HeLa cells (lanes 1 and 5), cell lines which expressed C468-A (lanes 2 and 6), SER-A (lanes 3 and 7) and C468-I (lanes 4 and 8) were infected with HSV-1(MOI = 0.9). Total RNAs were isolated from those cells 4 hours (A) or 10 hours (B) post infection. RNA samples (30 μg) were hybridized to the RNA probes containing the sequences of ICP4 and ICP47 (lanes 1–4) and to those containing ICP47 and TK mRNAs (lanes 5–8). The ICP47 mRNA was used as the internal loading control.

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