Table 1 Overall cleavage rates (Vmax(apparent)/Km(apparent))and binding affinities (Kd) in EGS-mediated cleavage reactions of pre-tRNASER or ICP4 mRNA by RNase P.

From: Inhibition of herpes simplex virus 1 gene expression and replication by RNase P-associated external guide sequences

Substrate

K m (μM)

Vmax (apparent)(pmol·min−1)

Vmax(apparent)/Km(apparent)(pmol·μM−1·min−1)

K d (μM)

pre-tRNASER

0.022 ± 0.071

0.069 ± 0.020

3.1 ± 0.5

 

ICP4 RNA (icp38)

+SER-A

0.60 ± 0.16

0.029 ± 0.010

0.048 ± 0.013

2.1 ± 0.5

+SER-I

ND

ND

<0.001

2.0 ± 0.5

+C468-A

0.29 ± 0.09

0.84 ± 0.36

2.9 ± 0.4

0.025 ± 0.005

+C468-I

ND

ND

<0.001

0.025 ± 0.005

  1. Kinetic parameters were determined with previously described protocols13,19. Binding affinities were measured without the presence of RNase P. The values shown are the average derived from triplicate experiments. “ND”: not determined.
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