Figure 4

Purification and identification of recombinant Tgf2TPases.

(a–c) SDS-PAGE analysis of S1-Tgf2TPase (a) and S2-Tgf2TPase (b) with N-terminal deletions, or of mutated L-Tgf2TPase^{D228N, E648Q} (c) expression from E. coli strain Rosetta 1 (DE3) by vector pET-28a(+). M, standard molecular marker. 0, without IPTG. 1, with IPTG. 2, purification from eluted peak of Ni²⁺-affinity column. (d–f) Mass spectrometry of S1-Tgf2TPase (d), S2-Tgf2TPase (e) or L-Tgf2TPase^{D228N, E648Q} (f) collected from eluted peak of Ni²⁺-affinity column. The confirmed fragments of Tgf2TPase are indicated in red, with the corresponding peptide sequences listed.