Figure 5
DNA-binding activity of S1-, S2- or L-Tgf2TPases analyzed by size exclusion chromatography.
(a) Elution profiles of the L50 double-stranded DNA probe alone. (b,c) Elution profiles of S1-Tgf2TPase alone (b) or a mixture of S1-Tgf2TPase and L50 (c). (d,e) Elution profiles of S2-Tgf2TPase alone (d) or a mixture of S2-Tgf2TPase and L50 (e). (f,g) Elution profiles of L-Tgf2TPase alone (f) or a mixture of L-Tgf2TPase and L50 (g). (h,i) Elution profiles of L-Tgf2TPaseD228N, E648Q alone (h) or a mixture of L-Tgf2TPaseD228N, E648Q and L50 (i). Based on elution times, peak 1 corresponds to the L50 probe and peaks 2 and 3 represent Tgf2TPase dimers and monomers, respectively. Peaks 4 and 5 are new multimeric complexes formed from presumptive binding of the L50 probe and the L-Tgf2TPase.
