Figure 6

Microscopy images of EGFP expression in zebrafish embryos after microinjection of S1-, S2- or L-Tgf2TPase with donor construct pTgf2-EF1α-EGFP at the 1-2-cell stage.

Light (a–c, g–i, m–o, s–u) and fluorescence (d–f, j–l, p–r, v–x) microscopy images of zebrafish embryos were shown at 12 hpf (a, d, g, j, m, p, s and v), 24 hpf (b, c, h, k, n, q, t, and w) and 96 hpf (c, f, i, l, o, r, u, and x). Panels a–f are embryos treated with L-Tgf2TPase, panels g-l were treated with S1-Tgf2TPase, panels m-r were treated with S2-Tgf2TPase and panels s-x injected with donor plasmid pTgf2-EF1α-EGFP alone as controls.