Figure 7
Exogenous Tgf2 end regions and the surrounding insertion site sequences in the genome of 3 month old adult zebrafish.
(a) Sequences of Tgf2 end regions in the donor construct pTgf2-EF1Ī±-EGFP. (b) Insertion sequences from 5 of 78 individuals listed in Table 2, with precise insertion sites after coinjection with pTgf2-EF1Ī±-EGFP and L-Tgf2TPase. (c) Insertion sequences from all 5 samples in Table 2 with imprecise insertion sites after coinjection with pTgf2-EF1Ī±-EGFP and L-Tgf2TPase. Precise insertion copies are shown with gray background. Lower case letters indicate the Tgf2 end sequences. The 8ābp direct repeat signature of the target DNA associated with transposition is underscored. The surrounding sequences of the insertion sites are in italic. Triangles and inverted triangles represent deletions and insertions, respectively.
