Scientific Reports

Table 1 Transposition efficiencies of N-terminal truncated Tgf2TPases in zebrafish.

From: The N-terminal zinc finger domain of Tgf2 transposase contributes to DNA binding and to transposition activity

Injection batches	No. of embryos			No. of adults
Injection batches	Examined	EGFP- expressed	Expressed rate (%)	Survived	EGFP- insertion	Insertion rate (%)
pTgf2-EF1α-EGFP + S1-Tgf2TPase	88	32	36	30	7	23
pTgf2-EF1α-EGFP + S1-Tgf2TPase	130	70	58	64	10	16
	46	19	41	16	4	25
Average	–	–	43	–	–	21^b
pTgf2-EF1α-EGFP + S2-Tgf2TPase	70	17	24	17	4	23
pTgf2-EF1α-EGFP + S2-Tgf2TPase	110	37	34	35	7	20
	45	13	29	13	4	31
Average	–	–	29	–	–	25^b
pTgf2-EF1α-EGFP + L-Tgf2TPase	90	60	67	55	28	51
pTgf2-EF1α-EGFP + L-Tgf2TPase	90	62	69	60	36	60
	56	38	68	34	19	56
Average	–	–	68	–	–	56^c
pTgf2-EF1α-EGFP + L-Tgf2TPase^{D228N, E648Q}	85	20	23	18	2	11
pTgf2-EF1α-EGFP + L-Tgf2TPase^{D228N, E648Q}	93	21	23	19	1	5
	75	16	21	14	1	7
Average	–	–	22	–	–	7^a
Control	86	21	24	18	2	11
	95	25	26	22	1	4
	86	20	23	18	2	11
Average	–	–	24	–	–	8^a

Control is injected pTgf2-EF1α-EGFP plasmid only. Different letters is significant p < 0.01.

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