Table 2 Insertion accuracy and germline transmission of N-terminal truncated Tgf2TPases in EGFP-transgenic adult zebrafish at 3-month age.
Injection batches | No. of fish examineda | No. of fish with precise insertion sites | No. of fish with imprecise insertion sites | No. of fish with undetectable insertion sites | Total precise insertion sites | Total imprecise insertion sites | Precise insertion sites/total insertion sites (%) |
|---|---|---|---|---|---|---|---|
pTgf2-EF1Ī±-EGFPā+āS1-Tgf2TPase | 21 | 3 (2) | 14 (4) | 4 (1) | 4 | 15 | 21 |
pTgf2-EF1Ī±-EGFPā+āS2-Tgf2TPase | 15 | 2 (2) | 10 (4) | 3 (0) | 3 | 12 | 20 |
pTgf2-EF1Ī±-EGFPā+āL-Tgf2TPase | 83 | 78 (71) | 5 (2) | 0 | 136 | 7 | 95** |
pTgf2-EF1Ī±-EGFPā+āL-Tgf2TPaseD228N, E648Q | 4 | 0 | 0 | 4 (1) | 0 | 0 | 0 |
Control | 5 | 0 | 1 (1) | 4 (0) | 0 | 1 | 0 |
