Figure 2 | Scientific Reports

Figure 2

From: TLR9-ERK-mTOR signaling is critical for autophagic cell death induced by CpG oligodeoxynucleotide 107 combined with irradiation in glioma cells

Figure 2

3-MA reverses the radiosensitizing effect of CpG ODN107 in U87 cells.

(a) 3-MA reversed the radiosensitizing effect of CpG ODN107. Cells were treated with or without of 3-MA (4 mM) for 2 h and then incubated with CpG ODN107 (10 μg/mL) for 12 h, before treatment with or without 5 Gy of irradiation. After incubation for another 24 h, the cells were collected for MTT assay. **P < 0.01 vs. IR; ††P < 0.01 vs. CpG 107 + IR. Error bars represent the mean ± S.D. (n = 5). (b) 3-MA reversed the autophagy-related proteins expressions. The cells were treated as described as (a), except the cells were collected for western blotting assay. In figures, CpG ODN107 was abbreviated as CpG 107; irradiation was abbreviated as IR.

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