Figure 3

CpG ODN107 combined with local radiotherapy induces autophagosome formation of tumor cells in nude mice with orthotopic transplantation tumor.
(a) The autophagosomes formation observed by TEM. Autophagosomes (marked by arrows) were presented as rounded vacuolar structures with double or multiple membranes surrounding the cytoplasmic contents. (b) Immunohistochemical analysis of p62 protein expression. p62 protein (brown) was expressed in the cytoplasm. In figures, CpG ODN107 was abbreviated as CpG 107; radiotherapy was abbreviated as IR.