Figure 5
CpG ODN107+ irradiation has different influences on MAPKs in U87 cells.
(a) ERK, JNK, P38 proteins expressions in U87 cells. Cells were treated with 10 μg/mL of CpG ODN107 for 12 h and then treated with or without 5 Gy of irradiation. After incubation for another 24 h, the cells were collected for western blotting assay. (b) The effects of U0126, an inhibitor of ERK, on LC3 and p62 protein expression. Cells were pretreated with U0126 (10 μM) for 2 h and then incubated with CpG ODN107 (10 μg/mL) for 12 h with or without 5 Gy of irradiation. After incubation for another 24 h, the cells were collected for western blotting assay. (c) The effect of U0126 on the cell viability. The cells were treated as described as (b), except the cells were collected for MTT assay. Error bars represent the mean ± S.D. (n = 5). **P < 0.01 vs. IR; ††P < 0.01 vs. CpG ODN107 + IR. In figures, CpG ODN107 was abbreviated as CpG 107; irradiation was abbreviated as IR.
