Figure 7
CpG ODN107 + irradiation upregulates TLR9, p-ERK expression and downregulates p-mTOR expression in CHG-5 and U251 cells.
(a) Cells were treated with 10 μg/mL of CpG ODN107 for 12 h and then treated with or without 5 Gy of irradiation. After incubation for another 24 h, the cells were collected for western blotting assay. (b) The effect of TLR9 siRNA on TLR9, p-ERK, p-mTOR and LC3 proteins expressions. Cells were transfected with control siRNA or TLR9-specific siRNA. All of transfection was performed for 24 h prior to treatment with 10 μg/mL of CpG ODN107 combined with 5 Gy of irradiation. After incubation for another 24 h, the cells were collected for western blotting assay. In figures, CpG ODN107 was abbreviated as CpG 107; irradiation was abbreviated as IR.
