Figure 2

Restraint of miR-200 family affects cell cycle and apoptosis.

The DMPCs were transfected with microRNA inhibitor negative control or combination of miR-200a/200b/429 inhibitors and then grouped as NC inhibitor, miR-200a/200b/429 inhibitors-DMPC, DMPC and MPC, respectively. (a) Cell-cycle distribution of podocytes were detected by a flow cytometer. (d) Cells transfected as in (a) were suspended and subjected to Annexin V-FITC/PI analysis. Dot plots show intensity of Annexin V-FITC fluorescence on the X-axis and PI fluorescence on the Y-axis. Lower left quadrant (LL): living cells (Annexin V⁻/PI⁻), Upper left quadrant (UL): necrotic cells (AnnexinV⁻/PI⁺), Upper right quadrant (UR): late apoptotic cells and necrotic cells (Annexin V⁺/PI⁺), Lower right quadrant (LR): early apoptotic cells (Annexin V⁺/PI⁻). Statistical result of Annexin V-positive apoptotic cell rate (LR + UR) was shown in (b). (e) Morphological validation of cell apoptosis was detected by TUNEL assay. The arrows showed the apoptotic cells (fluorescent-FITC, green). The intact figure showed a costaining with DAPI in the supplementary data (Fig. S1). Statistical result of TUNEL-positive apoptotic cell rate was shown in (c). The data are the average of three independent experiments and are shown as the mean ± S.D. Statistically significant differences between two groups was indicated by ***P < 0.001.