Figure 6

Over-expression of RSAD2 combining with restraint of miR-200 family revealed inhibition of cell differentiation and proliferation.

The DMPCs were co-transfected microRNA inhibitor negative control or combination of miR-200a/200b/429 inhibitors with pEGFP-RSAD2 plasmid and then grouped as NC Inhibitor, miR-200s Inhibitors + RSAD-DMPC, miR-200s Inhibitors-DMPC, DMPC and MPC, respectively. (a) Cell-cycle distribution of the podocytes were detected by a flow cytometer. (b) Nephrin (green) of podocytes was detected after 48 h. DAPI staining was used to detect nuclei and is merged with Nephrin in their respective panels. The scale bar represents 20 μm. (c) Cell proliferation were analysed by WST-8 assay at 48 h. (d) Corresponding histogram of Nephrin protein expression in western blot assay. A cropped blot was shown above the histogram and the corresponding full-length blot was shown in the supplementary data (Fig. S6). Data are means ± S.D. Statistically significant differences are indicated (**P < 0.01, ^##P < 0.01).