Figure 3: Deletion of M1-motif or M2-motif. | Scientific Reports

Figure 3: Deletion of M1-motif or M2-motif.

From: Newly identified motifs in Candida albicans Cdr1 protein nucleotide binding domains are pleiotropic drug resistance subfamily-specific and functionally asymmetric

Figure 3

(A) The strategy for constructing variants deleted of the entire NBD1 motif (M1-Del) or NBD2 motif (M2-Del). Deletions are represented by red lines. (B) Western blot and confocal microscopy images showing expression in a PM fraction and overall cellular localization of WT, M1-Del and M2-Del Cdr1p-GFP. Immunodetection of GFP-tagged Cdr1p and its variants was performed using HRP-labeled anti-GFP antibody. (C) Drug resistance profile of yeast strains overexpressing WT Cdr1p and deletion mutants M1-Del and M2-Del were determined using agarose-based drug susceptibility assays as described in Methods section. (D) R6G transport, (E) NR transport and (F) ATPase activities of WT and M1-Del and M2-Del Cdr1p variants. Efflux and ATPase activities were determined as described in Fig. 2. Mutant variants showing a significant difference compared to WT Cdr1p are marked with an asterisk (*). Results are means of at least 3 independent experiments. Error bars represent standard deviation.

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