Figure 1: The Cdx2 TEE is inactive in the postimplantation embryo and is progressively inactivated in blastocyst outgrowths.
From: Distinct mechanisms regulate Cdx2 expression in the blastocyst and in trophoblast stem cells
(a) Diagram of Cdx2 expression at blastocyst and postimplantation stages (red). (b) Expression of Cdx2 at E6.5 is limited to the extraembryonic ectoderm (ExE). (c) β-galactosidase staining in the TEE–lacZ reporter line at E6.5, showing lack of reporter activity. (d) CDX2 expression (green) and TEE activity (red) in a E7.5 embryo of the TEE-mRFP line. CDX2 and mRFP were detected by immunohistochemistry with anti-CDX2 and anti-DsRed antibodies. Nuclei are counterstained with DAPI. CDX2 is present in extraembryonic tissue, while TEE activity is absent. (e) β-galactosidase staining in the TEE-lacZ reporter line at blastocyst stage and (f) in two outgrowths of the same line (#6 and #19). Scale bar, 110 μm (b–d), 10 μm (e), and 50 μm (f).
