Figure 4: A novel regulatory element drives Cdx2 expression in the TE, TS and a subset of extraembryonic cells. | Scientific Reports

Figure 4: A novel regulatory element drives Cdx2 expression in the TE, TS and a subset of extraembryonic cells.

From: Distinct mechanisms regulate Cdx2 expression in the blastocyst and in trophoblast stem cells

Figure 4

(a) UCSC genome browser view of the Cdx2 region (mm9; chr5:148,081,494-148,134,458) showing (from top to bottom) ENCODE tracks of histone modifications for active enhancers (H3K4me1 and H3K27ac) in the placenta, small intestine and ES cells. The TEE and fragments #1 and #2 are highlighted in grey. Fragment #3 is highlighted in red. (b) mRFP (red) activity driven by fragment #3 in blastocysts, showing co-localization with CDX2 (green). Nuclei (blue) were stained with DAPI. (c) Fragment #3 is active in TS cells. Expression driven by fragments #1 and #3 were compared to Oct4DE. Data are means ± s.e.m. n = 6. **p < 0.01 (Student’s t-test). (d) LacZ reporter activity driven by fragment #3 in transient transgenic embryos. (d’) Sagittal section of the embryo shown in (c). ExE, extraembryonic ectoderm; EcC, ectoplacental canal. Scale bar, 10 μm (b), 110 μm (d,d’).

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