Figure 1: Sleep effects of MK-1064 30 mg/kg are OX2R-dependent.
From: Orexin 2 Receptor Antagonism is Sufficient to Promote NREM and REM Sleep from Mouse to Man
(a) The mean time spent in active wake, light sleep (LS), SWS, and REM sleep during 30-min intervals was determined in wild-type (left panel; n = 5) and OX2R knockout (right panel; n = 7) mice following administration of MK-1064 30 mg/kg (open circles) relative to vehicle (20% vitamin E TPGS, orally; closed circles) during the late active phase (ZT: 20:00). Time points at which significant differences exist between vehicle and MK-1064 responses are indicated by grey vertical lines (*, **, ***p < 0.05, 0.01, 0.001, respectively; linear mixed-effects model for repeated measures). (b) Mean within-subject difference ± standard error of the mean of time spent in active wake, LS, SWS, and REM for 2 hours following oral treatment with MK-1064 (30 mg/kg) versus vehicle (20% vitamin E TPGS) in wild-type and OX2R knockout mice is shown (**p < 0.01, one sample t-test for repeated measures compared with vehicle).
