Figure 3
From: Enhancement of anti-STLV-1/HTLV-1 immune responses through multimodal effects of anti-CCR4 antibody
Recombinant vaccinia viruses expressing sTax and SBZ induce immune responses and suppress sTax-expressing cells.
(a) STLV-1-infected JM08 and JM09 were vaccinated with rVV-sTax M22 or rVV-SBZ LL/AA, respectively. CD4- or CD8- depleted PBMCs were analyzed by IFN-γ ELISPOT assay using sTax or SBZ pooled peptides. Data shown are the total number of spots from pooled peptides (P1–P4) stimulation. (b) STLV-1 proviral load in the vaccinated monkeys. (c) Decrease in the number of sTax-expressing cells after rVV vaccination. PBMCs from monkeys were depleted of CD8+ T cells and cultured for 24 hours and sTax expressing CD4+ T cells were detected. (d) High throughput sequencing of STLV-1 integration sites in infected JMs before (0 weeks) and after (40 weeks) vaccination. The number of cells of each STLV-1 clone per 1,000,000 PBMCs was calculated. STLV-1 infected clones that grew more populous and had more than 500 copies at 40 weeks are shown as red lines.
