Figure 5 | Scientific Reports

Figure 5

From: miR-139-5p Inhibits the Epithelial-Mesenchymal Transition and Enhances the Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating BCL2

Figure 5

miR-139-5p directly targeted BCL2 in colon cancer cells.

(A) Schematic representation of the miR-139-5p putative binding sites in the 3′-UTR of BCL2 mRNA and the mutations introduced into the BCL2 3′-UTR regions. (B) BCL2 mRNA and protein expression were determined by RT-PCR and Western blotting. (C) Wild-type (WT) or mutated (MUT) BCL2 reporter constructs were co-transfected into HCT116 and SW620 cells or controls. The relative luciferase activities were measured. (D) Representative images of the IHC staining of BCL2 in colorectal cancer patients. (E) miR-139-5p expression levels were inversely correlated with BCL2 expression in colorectal cancer samples. *P < 0.001. (E) Representative images of the IHC examination of BCL2 in mouse tumours from miR-139-5p KO and WT mice.

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