Figure 4: Upregulation of p75NTR and effect of local proBDNF injection on inflammatory reaction in mice.
From: Peripheral Brain Derived Neurotrophic Factor Precursor Regulates Pain as an Inflammatory Mediator
(A) Representative Western blot of p75NTR and sortilin (a) and the semi-quantitative analysis of their expression (b and c) after formalin intra-plantar injection (***p < 0.001 versus control, one-way ANOVA followed by Dunnett’s Multiple Comparison post hoc test, n = 3 per group). (B) p75NTR immunohistochemistry in the local tissue at 3 h after 5% formalin intra-plantar injection. Image in lower panel is the higher magnification of box in the upper panel. Scale bar, 50 μm, 3 replicates, n = 3 per group. (C) H&E staining of local tissue 3 h after exogenous proBDNF (1 μg) injection, Scale bars, 100 μm, 3 replicates, n = 3 per group. (D) Representative Western blot of local cytokines IL-1β, IL-6 and TNF-α expression and their semi-quantitative analysis at 3 h and 24 h after 1 μg exogenous proBDNF intra-plantar injection (*p < 0.05, **p < 0.01, ***p < 0.001 versus control, one-way ANOVA followed by Dunnett’s Multiple Comparison post hoc test, n = 4 per group). (E) Time course of IL-1β, IL-6 and TNF-α gene expression 3h after 0.25 μg and 1 μg proBDNF injection (*p < 0.05, **p < 0.01, ***p < 0.001 versus control, one-way ANOVA followed by Dunnett’s Multiple Comparison post hoc test, n = 3 per group). Data bars represent mean ± s.e.m.
