Figure 3

High exogenous IAA complements the lateral root growth defect of pyl8-1pyl9 on ABA-containing medium.

(a) Root architecture of Col-0, pyl8-1 and pyl8-1pyl9 mutants under ABA and ABA with IAA treatment. Root architecture of seedlings was documented at 7 dpt. Seedlings were transferred at 4 dpg to the control medium (1/2 MS, 1% sucrose) (upper panel) or medium supplemented with 5 μM ABA (middle panel) or 5 μM ABA and 100 nM IAA (bottom panel). Bar, 1 cm. (b) Average lateral root length of Col-0, pyl8-1 and pyl8-1pyl9 mutants grown with or without ABA treatment or ABA plus IAA treatment. Lateral root length of seedlings grown on ABA-containing medium was measured at 7 dpt. The concentrations of ABA and IAA in the medium are as indicated. Error bars indicate s.e.m. (n = 25 seedlings, 5 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test. (c) The lateral root length of seedlings was measured at the indicated days after transfer to media with or without ABA or ABA plus IAA. Error bars indicate s.e.m. (n = 25 seedlings, 5 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test. Asterisks indicate comparison between pyl8-1 and pyl8-1pyl9.