Figure 4
From: The ABA receptor PYL9 together with PYL8 plays an important role in regulating lateral root growth
Induced expression of PYL9 increases the average lateral root length and shortens the quiescent phase.
(a) Root architecture of pRD29A:PYL9#13, pRD29A:PYL9#27 and Col-0 under ABA treatment. Seedlings were transferred at 4 dpg to the control medium (1/2 MS, 1% sucrose) (upper panel) or medium supplemented with 1 μM ABA (bottom panel). Root architecture of seedlings was documented at 7 dpt. Bar, 1 cm. (b) The lateral root length of Col-0, pRD29A:PYL9#13 and pRD29A:PYL9#27 was measured at the indicated days after transfer to media supplemented with ABA. Seedlings were transferred at 4 dpg. Error bars indicate s.e.m. (n = 25 seedlings, 5 independent experiments). **P < 0.01, ***P < 0.001, Student’s t test. Asterisks indicate comparison between Col-0 and pRD29A:PYL9#13, Col-0 and pRD29A:PYL9#27. (c) Pie charts of the percentage of quiescent lateral roots for the indicated number of days on seedlings on control medium and medium with ABA (n = 25 seedlings, 5 independent experiments). The proportion of quiescence less than 3 days of Col-0 is significantly less than that of pRD29A:PYL9#13 and pRD29A:PYL9#27, respectively (p-value < 0.001, one-tailed binomial test).
