Figure 7
From: The ABA receptor PYL9 together with PYL8 plays an important role in regulating lateral root growth
PYL9 directly regulates MYB77 transcriptional activity in vivo.
(a) PYL9 enhances the ability of MYB77 to activate IAA19 expression in Col-0 protoplasts. PYL9, MYB77, IAA19-LUC and ZmUBQ-GUS were co-expressed in protoplasts. IAA19-LUC was used as the auxin-responsive reporter. ZmUBQ-GUS was used as the internal control. After transfection, protoplasts were incubated for 12 h under light in the absence of hormone (open bars) or in the different combinations of 5 μM ABA and 1 μM IAA. Error bars indicate s.e.m. (n ≥ 3 experiments). **P < 0.01, ***P < 0.001, Student’s t test. (b,c) ChIP-PCR and ChIP-quantitative-PCR for IAA7 promoter. The 500-bp region of the translational start of IAA7 contains MBSI elements recognized by MYB77 and MYB44. pPYL9:PYL9-YFP-HA was treated with ABA and IAA before conducting ChIP assay. Col-0 was used as control. Numbers are folds compared to Col-0 adding anti-HA. Error bars indicate s.e.m. (n = 3 experiments). *P < 0.05, Student’s t test.
