Table 1 Sequence of primers used for amplification of partial cDNA, 3′ and 5′ RACE of topmouth culter TGF-β1 and qPCR analysis for cTGF-β1,TNF-α and IL-1β.
Primer | Sequence (5′–3′) | Usage |
|---|---|---|
T-1 | GAYCTGGRNTGGAARTGGAT | TGF-β1 partial |
GARGCBATTMGRRGHCAGAT | ||
T-2 | GCMGAKGCWCCDGGRTTGTG | TGF-β1 partial |
GTTTTCAGCATTCCAGATGTAGGTG | ||
5′RACE | CCTTGTGTTGTCTTCC | TGF-β1 5′RACE |
GACTGCAGGTACGCAACATGG | ||
GAGTATTTGTTCTCAGCATTCC | ||
3′RACE | GTGCATCTGCTCAGCCATGTTGCGTACCT | TGF-β1 3′RACE |
CCTGCAGTCCTCGACCCTCTACCAATTCT | ||
T-y | CAGGATCCGATGTTGGCACTGATGTAACTTG | TGF-β1 expression |
GACCTCGAGACTGCACTTGCAGCTCCTCAC | ||
T-q | GGAGGTGCACAAGTTCATTAT | TGF-β1 qPCR |
GGCAGATATGATGGTGAAGCC | ||
TNF-q | ACTGTCTCCTTCACGCTCCA | TNF-α qPCR |
CTGTGAGGTGCCATTCGCTT | ||
IL-q | ACGCAGGCCGGAGCCTCTGT | IL-1β qPCR |
CCTGTAGATGAGGCGGCTGTC | ||
β-actin | TGCGTGACATCAAGGAGAAG | internal control |
GCTGGAAGGTGGACAGAGAG |
