Figure 4: Neighbour-joining phylogenetic tree based on amino acid sequences of bifunctional diterpene and sesterterpene synthases, farnesyl pyrophosphate synthases and hexaprenyl pyrophosphate synthases.
From: Sesterterpene ophiobolin biosynthesis involving multiple gene clusters in Aspergillus ustus
The terpene synthases involved in ophiobolin biosynthesis are found in three clusters. light orange: bifunctional di(sester) terpene synthases; light purple: hexaprenyl pyrophosphate synthases; light blue: farnesyl pyrophosphate synthases. The tree was generated based on the Kimura 2-parameter matrix in MEGA software (version 5.0). Numbers at nodes are bootstrap values obtained using bootstrapping with 1000 repetitions. The scale bar corresponds to 0.2 substitutions per nucleotide position. Aspergillus clavatus ophiobolin F synthase (AcOS A1C8C3), Phomopsis amygdali phomopsene synthase (PaPS AB254159), Phomopsis amygdali fusicoccadiene synthase (PaFS AB267396), Alternaria brassicicola fusicoccadiene synthase (AbFS C9K2Q3), Ornithogalum saundersiae farnesyl pyrophosphate synthase (OsaFPPS KF509889), Rhopalosiphum padi isoprenyl diphosphate synthase (RpFPPS1 HQ850372 and RpFPPS2, HQ850373), Euphorbia pekinensis farnesyl diphosphate synthase (EpFPPS FJ755465), Sulfolobus Solfataricus Hexaprenyl Pyrophosphate Synthase (SsHexPPS 2AZK_B), Micrococcus luteus heterodimeric hexaprenyl diphosphate synthase (MlHexPPS 3AQB_B), Aspergillus oryzae hexaprenyl pyrophosphate synthase (AoHexPPSXP_001824458), Neosartorya fischeri hexaprenyl pyrophosphate synthetase (NfHexPPS XP_001263839).
