Figure 5: GC-MS profiles of the products of in vitro enzymatic reactions.
From: Sesterterpene ophiobolin biosynthesis involving multiple gene clusters in Aspergillus ustus
The chromatograms of Au8003 reaction product ophiobolin F extracted at m/z 358 (a): (I) GGPP+IPP, (II) FPP+IPP, (III) GPP+IPP, (IV) DMAPP+IPP and (V) IPP, and MS Spectra of peak 1 (b); chromatograms of Au13192 reaction product veridiene extracted at m/z 272 (c): (I) FPP+IPP, (II) GPP+IPP, (III) DMAPP+IPP, (IV) IPP and MS Spectra of peak 2 (d); chromatograms of phosphatase hydolysate of Au6298 reaction product FPP extracted at m/z 222 (e): (I) DMAPP+IPP, (II) IPP only and MS Spectra of peak 3 (f).
