Figure 2: Birth of a transgenic (tg) cow with the YFP gene via Sleeping Beauty (SB) and its analysis. | Scientific Reports

Figure 2: Birth of a transgenic (tg) cow with the YFP gene via Sleeping Beauty (SB) and its analysis.

From: Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing

Figure 2

(a) After 60 days of embryo transfer, pregnancy was confirmed by ultrasonography. The calf was delivered without assistant (b) and grew to 5-months (c) and 16 months (d) old without any health issue. (e) When ultraviolet light was exposed to nose of tg cattle, YFP expression was found (arrow). To determine YFP expression in primary skin and endometrial cells, the cells were cultured and captured by confocal image equipment ((f-1) skin cells from a wild type, (f-2) skin cells from a tg cattle, (f-3) endometrial cells from a tg cattle, upper: brightness, lower: fluorescence). The primary skin cells from tg or non-tg were reprogrammed and developed into blastocysts (f-4) blastocysts from skin cells of non-tg cattle, (f-5) blastocysts from skin cells of the tg cattle; upper: brightness, lower: fluorescence). The tg integration was confirm by PCR (g) and sequencing (h). 1: Molecular maker, 2: Wild type cattle, 3: Positive control (DNAs), 4: Blood from tg cattle, 5: Ear tissues from tg cattle, 6: Placenta from tg cattle, 7: Negative controls. Gel image was cropped and original image was seen in Supplementary Figure 8.

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