Figure 4: Birth of a transgenic (tg) cattle with the pβ-casein-hIL2-pCAG-GFP gene via Piggybac (PB) and its analysis. | Scientific Reports

Figure 4: Birth of a transgenic (tg) cattle with the pβ-casein-hIL2-pCAG-GFP gene via Piggybac (PB) and its analysis.

From: Efficient generation of transgenic cattle using the DNA transposon and their analysis by next-generation sequencing

Figure 4

(a) After 45 days of embryo transfer, pregnancy was confirmed by ultrasonography. (b) The calf was delivered without any assistance and grew up to 2 months. Analyzing the calf without ultraviolet light, GFP expression was observed in the eyes (c) and nose (d). The tg cattle have been grown to 5 months old without any health issue (e). When ultraviolet light was exposed to the head, GFP expression was strongly observed (f). To know GFP in skin cells, the primary skin cells from the tg cattle were isolated and cultured. In over 99% of cells, GFP expression were observed ((g) brightness; (g`) fluorescence). The transgene integration was confirmed by genomic DNA PCR ((h) 1: Molecular maker, 2: Wild type cattle, 3: Blood from tg cattle, 4: Positive control (DNAs), 5: Negative control) and RT-PCR using primary cells ((i) 1: cDNA from Wild type cattle, 2: cDNA from tg cattle, 3: Negative control). Gel image was cropped and original image was seen in Supplementary Figure 8.

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