Figure 4
From: Quantifying promoter activity during the developmental cycle of Chlamydia trachomatis
Identifying the determinants of ompA P3 region.
(a) Sequences of P3 and its derivatives tested in this study. (b) Autoradiogram of a denaturing acrylamide gel showing the transcript products of in vitro transcription assays with P3 or its derivatives. σ66R24 RNAP holoenzyme (left panel) or σ28 RNAP holoenzyme (right panel) were used. (c) An autoradiogram of a denaturing acrylamide gel showing ompA P3 transcripts produced in the transcription assay. E. coli σ70 RNAP holoenzyme was used. The amounts of transcripts were determined by densitometry using ImageJ49 and are shown relative to WT P3, which is set at 100%, as indicated at the bottom of (b,c). Note: P3 is transcribed by both σ66R24 RNAP and σ70 RNAP, but not σ28 RNAP.
