Figure 7: In vivo pro-apoptotic properties of climacostol in mice bearing melanoma allografts. | Scientific Reports

Figure 7: In vivo pro-apoptotic properties of climacostol in mice bearing melanoma allografts.

From: Climacostol reduces tumour progression in a mouse model of melanoma via the p53-dependent intrinsic apoptotic programme

Figure 7

Subcutaneous B16-F10 melanoma allografts were excised from mice at day 16 of treatment (from day 0 - every 3–4 days) with vehicle (control) or climacostol (600 μg/ml). (a) Percentage of viable cells inside the tumours as evaluated by Trypan Blue staining. (b) Representative immunofluorescence imaging of cleaved Caspase 3. DAPI (blue) was used for nuclei detection. Scale bar: 20 μm. (c,d) Western blot analysis of cleaved Caspase 3 and p53 expression, respectively. GAPDH was used as the internal standard. (e) mRNA levels of p53 and its target genes p21, Noxa and Puma, as measured by real-time PCR. Data are expressed as the fold change over control (set as 1). Images and data represent the results obtained from 3 animals per experimental group. *p < 0.001 and **p < 0.0001 vs the respective control. (f) Schematic picture depicting cell death mechanisms of climacostol in melanomas.

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