Figure 3
Overexpression of hTau activates calpains.
The 7 DIV hippocampal neurons were infected with lenti-mCherry-hTau virus or the vector for 5 days, then the cell extracts were prepared for Western blotting (a,b) or the calpain activity assay. (c) Overexpression of hTau increased total levels of calpain-1 (two-sample unpaired t test, t4 = 3.933, p = 0.0043), calpain-2 (two-sample unpaired t test, t4 = 4.903, p = 0.0017), the activated calpain-1 (aCalp-1) (two-sample unpaired t test, t4 = 3.724, p = 0.0074), the activated calpain-2 (aCalp-2) (two-sample unpaired t test, t4 = 5.143, p = 0.0068) and the calpain activity (two-sample unpaired t test, t10 = 9.639, p < 0.0001) with a decreased level of cleaved caspase-3 (two-sample unpaired t test, t4 = 5.456, p = 0.0003). The data were from at least three independent experiments and were expressed as mean ± SEM, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs vector.
