Figure 5
Inhibition of calpain-2 rescues the hTau-induced inhibition of α4 nAChR currents in hippocampal neurons.
(a) The cultured hippocampal neurons (7 DIV) were infected by lenti-mCherry-hTau virus or the vector. After 3 days, the neurons were treated with 10 μM mCalp-I for 48 h and then the α4 nAChR agonist RJR2403 (1 mM, 50 ms) was puffed to elicit α4 current recorded by whole-cell patch clamp. (b) The amplitude of currents were analyzed (two-way ANOVA, factor infection: F1,24 = 6.492, p = 0.0177; factor drug: F1,24 = 4.380, p = 0.0471; infection × drug: F1,24 = 6.097, p = 0.0210; Tukey’s post hoc test). At least 7 neurons from each group were recorded and the data were expressed as mean ± SEM. **p < 0.01 vs Vec+Vehicle; #p < 0.05, vs hTau+Vehicle.
