Figure 6
Overexpression hTau in hippocampal neurons increases the level of intracellular basal calcium.
The 7 DIV hippocampal neurons were infected with lenti-mCherry-hTau virus or the vector for 5 days and then the intracellular basal calcium level was measured by Fluo-3 AM expressed as F/F0. (a) The representative imaging in primary hippocampus neurons overexpressing with hTau and the control virus. (b) Cumulative distribution of basal [Ca2+]i showed higher basal calcium level in hippocampal neurons expressing hTau (n = 112) than in neurons expressing vector (n = 62). (c) The fluorescence intensity of intracellular basal calcium was quantitatively analyzed (two-sample unpaired t test, t172 = 9.232, p < 0.0001), F is the real time fluorescence intensity of Fluo-3 AM, F0 is the mean fluorescence intensity of the baseline recorded. The values of calcium signal were expressed by F/F0. Data were expressed as mean ± SEM. ****p < 0.0001 vs vector group.
