Figure 1: Microtubules in Drosophila macrophages. | Scientific Reports

Figure 1: Microtubules in Drosophila macrophages.

From: Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison

Figure 1

Microtubules were detected with primary and secondary antibodies, the latter coupled to Alexa Fluor 488 for SIM and STED and Alexa Fluor 647 for SMLM. 100% available depletion laser power was used for STED. (a) Single optical section through the microtubule network close to the coverslip. Scale bar, 5 μm. The boxed areas are magnified in (b,c). In the STED image out of focus blur (arrow) and an artefact caused by high laser intensity (arrow head) are visible. (b) Region showing well separated microtubules. Scale bar, 0.5 μm. (c) Region with microtubule bundles. Scale bar, 0.5 μm (d) Results of FWHM measurements of 50 microtubules per super-resolution technique. (e) Representative normalised line profiles for each super-resolution technique. Close to the microtubule, intensity values for SIM can dip into negative values, which is an artefact caused by the SIM reconstruction.

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