Figure 4: Transport vesicles in COS7 cells. | Scientific Reports

Figure 4: Transport vesicles in COS7 cells.

From: Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison

Figure 4

Sec31A was detected with primary and secondary antibodies, the latter coupled to Alexa Fluor 488 for confocal imaging, SIM and STED and Alexa Fluor 647 for SMLM. 100% available depletion laser power was used for STED. (a,c) Overview of vesicles close to the nucleus in a single optical section. Boxed areas are magnified in (b,d). Scale bar, 5 μm. (b,d) Vesicles of various sizes with a little out of focus background visible as weak, small foci in STED. Scale bar, 0.5 μm.

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