Figure 4 | Scientific Reports

Figure 4

From: Rhomboid intramembrane protease RHBDL4 triggers ER-export and non-canonical secretion of membrane-anchored TGFα

Figure 4

RHBDL4 enhances ER-to-Golgi trafficking of various type I membrane proteins.

(a) RHBDL4-induced secretion of higher MW form is also observed for ectopically expressed CD44 (to a lower extend also by RHBDL1). (b) EndoH and PNGaseF treatment of cell extracts shows that higher molecular weight forms of CD44 (black arrow) localize to the late secretory pathway, whereas the lower molecular weight form (grey arrow) is ER-resident. Dot, deglycosylated form. (c) Cell-based rhomboid assay as in (a) using Drosophila Spitz. (d) RHBDL4-induced shift of Spitz to a higher molecular weight form mimics Star-mediated ER-to-Golgi transport. Asterisk, EndoH-insensitive fraction of Spitz.

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