Figure 4
From: Discovery of a rhamnose utilization pathway and rhamnose-inducible promoters in Pichia pastoris
Expression profiles of heterologous genes driven by PLRA3 and PLRA4 in P. pastoris.
(A) Schematic representation of plasmid pGHLRA3α for heterogeneous gene expression in P. pastoris. (B) β-galactosidase activity under the control of PLRA3, PLRA4 and PGAP at various time points. (C) Production of recombinant β-galactosidase using PLRA3, PLRA4 and PGAP for various durations. (D) Green fluorescence under the control of PLRA3, PLRA4 and PGAP; ‘C’ represents P. pastoris GS115, which was used as a control.
