Figure 4
Validation of RNA-seq data revealed by FISH of DEG in dissected tissues Confocal pictures showing the expression of genes up-regulated after DEGs analysis.
Green: riboprobes; blue: nuclei (DAPI). For each selected gene, FISH pictures are shown for all three collected tissues (rows of the panel). Each column shows a single gene expression pattern (gene names at the top of the columns); difference in relative expression of transcripts is indicated within parentheses. White dashed line delineates, for each tissue, the portion of collected sample for RNA sequencing. Arrowheads indicate areas of gene expression (absent when FISH experiment did not allow transcript detection). Red asterisks indicate non-specific signal due to the extracorporeal matrix, the tunic. (a), (a’), (b’), (c’) and (f’) shows expression in the budlet inner epithelium. (d’), (d”), (e’) and (e”) shows expression in the mantle of the primary bud (muscle fibers). All pictures are shown with the same orientation, relatively to the primary bud body axes, as represented in picture (a). Scale bar 40 μm.
