Figure 1

SspA-1 is secreted in a T4SS-dependent manner.
(A) Western blot analysis comparing the levels of SspA-1 in the supernatants and cell pellets of the designated S. suis 2 strains. RNA polymerase subunit RpoB was used as loading control. (B) sspA-1 transcripts quantified through real-time PCR. N. S. indicates no significant difference (P > 0.05) between S. suis 2 wild-type stain 05ZYH33 and T4SS-deficient mutant (ΔvirD4–89K).